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Adult mesenchymal stem cells (MSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) are distinct cell populations with different gene expression patterns. To elucidate the role of BMSCs in neonatal and pediatric diseases, a comprehensive understanding of the biological properties of BMSCs is needed. We performed a comparison of MSC characteristics between pediatric and adult BMSCs. Cell proliferation, adipogenesis, osteogenesis, and transfection efficiency were studied in BMSCs from children. These BMSCs were similar to adult BMSCs in terms of their morphology, cell proliferation, and surface antigens, as well as the results of adipogenesis and osteogenesis. In addition, no difference in the expression of DNA methyltransferases was observed between pediatric and adult BMSCs, suggesting that the same methylation pattern is present in pediatric and adult BMSCs. Finally, the transfection efficiency of pediatric BMSCs was similar to that of adult BMSCs. These results demonstrate that BMSCs isolated from pediatric patients are similar to BMSCs isolated from adults and therefore may be used to explore the physiological properties of BMSCs.Q:
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